So for this latest work, have you successfully connected to your ECU using Guzzidiag? (sounds like a yes? or was that a while back?)
If so, then you know your laptop/driver/cable set up is working right. So it should be easy to get the reader going.
For the Reader, if you've never used it before, are you sure you downloaded the correct version for your bike?
If you have the right version, and good cables/driver, then the only thing left is picking the correct Com port. It would be the same com port number you used with Guzzidiag. That could have changed if you've done stuff to your laptop (like 6 months ago it could have been COM4 and now it could be COM5 if you've added extra stuff on your laptop).
Easiest way to check this ...
- don't have the cable connected to your laptop
- start the Reader, look at the list of com ports (let's say you see COM1-COM3)
- exit Reader
- connect your cable to your laptop, give it a few seconds to realized it is on there
- start the Reader again, look at the list of com ports ... the new one in that list is your cable (like now you'll see a COM4 or COM12, basically 1 more than before)